Danio rerio Oocytes for Eukaryotic In-Cell NMR.
Biochemistry
; 60(6): 451-459, 2021 02 16.
Article
em En
| MEDLINE
| ID: mdl-33534998
ABSTRACT
Understanding how the crowded and complex cellular milieu affects protein stability and dynamics has only recently become possible by using techniques such as in-cell nuclear magnetic resonance. However, the combination of stabilizing and destabilizing interactions makes simple predictions difficult. Here we show the potential of Danio rerio oocytes as an in-cell nuclear magnetic resonance model that can be widely used to measure protein stability and dynamics. We demonstrate that in eukaryotic oocytes, which are 3-6-fold less crowded than other cell types, attractive chemical interactions still dominate effects on protein stability and slow tumbling times, compared to the effects of dilute buffer.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oócitos
/
Ressonância Magnética Nuclear Biomolecular
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Biochemistry
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Estados Unidos