Two new steroidal sapogenins from Rohdea chinensis (synonym Tupistra chinensis) rhizomes and their antifungal activity.

Two novel acylated steroidal sapogenins, 3ß-acetoxy-1α, 2α, 4ß, 5α, 7α-pentahydroxy-spirost-25(27)-en-6-one (1) and (25S)-3α-acetoxy-1α, 2ß, 4α, 5α, 7α-pentahydroxyspirostan-6-one (2), together with two known steroidal sapogenins (3 and 4), were isolated from Rohdea chinensis rhizomes. Their structures were elucidated by nuclear magnetic resonance (NMR) and mass spectrometry (MS) data. In addition, the antifungal activities of the isolated compounds against Penicillium digitatum and Rhizopus stolonifera were evaluated. Compound 2 significantly inhibited R. stolonifera growth, which was comparable to the positive control (sodium benzoate and carbendazim). Compound 4 showed the highest potency to inhibit P. digitatum growth compared to other compounds.