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Effects of Uremic Toxins on the Binding of Aripiprazole to Human Serum Albumin.
Nishi, Koji; Sakurama, Keiki; Watanabe, Hiroshi; Maruyama, Toru; Yamasaki, Keishi; Otagiri, Masaki.
Afiliação
  • Nishi K; Faculty of Pharmaceutical Sciences, Sojo University.
  • Sakurama K; Faculty of Pharmaceutical Sciences, Sojo University.
  • Watanabe H; Graduate School of Pharmaceutical Sciences, Kumamoto University.
  • Maruyama T; Graduate School of Pharmaceutical Sciences, Kumamoto University.
  • Yamasaki K; Faculty of Pharmaceutical Sciences, Sojo University.
  • Otagiri M; DDS Research Institute, Sojo University.
Biol Pharm Bull ; 44(3): 437-441, 2021.
Article em En | MEDLINE | ID: mdl-33642552
We recently reported that aripiprazole (ARP), an antipsychotic drug, binds strongly to human serum albumin (HSA), the major drug binding protein in serum. It is known that uremic toxins that accumulate during renal disease affect the interaction between HSA and drug binding. In this study, the issue of how uremic toxins (indoxyl sulfate, indole acetic acid and p-cresyl sulfate) affect the binding of ARP to HSA was investigated. Equilibrium dialysis experiments revealed that all uremic toxins inhibited the binding of ARP to HSA although the inhibitory effects differed, depending on the specific uremic toxin. The potency of inhibition can be partially explained by the affinities of uremic toxins to HSA. Fluorescence displacement experiments suggested that ARP as well as all uremic toxins bind to site II of HSA. The inhibitory effects of the toxins on the binding of ARP for the drugs binding to the diazepam subsite are significantly larger, comparing with those for binding to arylpropionic acids subsite. Interestingly, induced circular dichroism (CD) spectra indicated that the spatial orientation of p-cresyl sulfate in the binding pocket is different from that for indoxyl sulfate and indole acetic acid. The limited findings obtained herein are important data in considering the effects of uremic toxins on the pharmacokinetics of ARP and the drugs that bind to site II on HSA, particularly drugs binding to diazepam binding site in site II.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ésteres do Ácido Sulfúrico / Antipsicóticos / Cresóis / Aripiprazol / Albumina Sérica Humana / Indicã / Ácidos Indolacéticos Limite: Humans Idioma: En Revista: Biol Pharm Bull Assunto da revista: BIOQUIMICA / FARMACOLOGIA Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ésteres do Ácido Sulfúrico / Antipsicóticos / Cresóis / Aripiprazol / Albumina Sérica Humana / Indicã / Ácidos Indolacéticos Limite: Humans Idioma: En Revista: Biol Pharm Bull Assunto da revista: BIOQUIMICA / FARMACOLOGIA Ano de publicação: 2021 Tipo de documento: Article