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Mass Spectrometry Imaging Reveals a Gradient of Cancer-like Metabolic States in the Vicinity of Cancer Not Seen in Morphometric Margins from Microscopy.
Woolman, Michael; Katz, Lauren; Gopinath, Georgia; Kiyota, Taira; Kuzan-Fischer, Claudia M; Ferry, Isabelle; Zaidi, Mark; Peters, Kaitlyn; Aman, Ahmed; McKee, Trevor; Fu, Fred; Amara-Belgadi, Siham; Daniels, Craig; Wouters, Brad G; Rutka, James T; Ginsberg, Howard J; McIntosh, Chris; Zarrine-Afsar, Arash.
Afiliação
  • Woolman M; Techna Institute for the Advancement of Technology for Health, University Health Network, 100 College Street, Toronto, Ontario M5G 1P5, Canada.
  • Katz L; Department of Medical Biophysics, University of Toronto, 101 College Street, Toronto, Ontario M5G 1L7, Canada.
  • Gopinath G; Techna Institute for the Advancement of Technology for Health, University Health Network, 100 College Street, Toronto, Ontario M5G 1P5, Canada.
  • Kiyota T; Department of Medical Biophysics, University of Toronto, 101 College Street, Toronto, Ontario M5G 1L7, Canada.
  • Kuzan-Fischer CM; Techna Institute for the Advancement of Technology for Health, University Health Network, 100 College Street, Toronto, Ontario M5G 1P5, Canada.
  • Ferry I; Department of Medical Biophysics, University of Toronto, 101 College Street, Toronto, Ontario M5G 1L7, Canada.
  • Zaidi M; Drug Discovery Program, Ontario Institute for Cancer Research, 661 University Avenue, Toronto, Ontario M5G 0A3, Canada.
  • Peters K; Arthur and Sonia Labatt Brain Tumor Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada.
  • Aman A; Developmental & Stem Cell Biology Program, The Hospital for Sick Children, 686 Bay Street, Toronto, Ontario M5G 0A4, Canada.
  • McKee T; Arthur and Sonia Labatt Brain Tumor Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada.
  • Fu F; Developmental & Stem Cell Biology Program, The Hospital for Sick Children, 686 Bay Street, Toronto, Ontario M5G 0A4, Canada.
  • Amara-Belgadi S; Department of Medical Biophysics, University of Toronto, 101 College Street, Toronto, Ontario M5G 1L7, Canada.
  • Daniels C; STTARR Innovation Centre, 101 College Street, Toronto, Ontario M5G 1L7, Canada.
  • Wouters BG; Techna Institute for the Advancement of Technology for Health, University Health Network, 100 College Street, Toronto, Ontario M5G 1P5, Canada.
  • Rutka JT; Department of Medical Biophysics, University of Toronto, 101 College Street, Toronto, Ontario M5G 1L7, Canada.
  • Ginsberg HJ; Drug Discovery Program, Ontario Institute for Cancer Research, 661 University Avenue, Toronto, Ontario M5G 0A3, Canada.
  • McIntosh C; Leslie Dan Faculty of Pharmacy, 144 College Street, Toronto, Ontario M5S 3M2, Canada.
  • Zarrine-Afsar A; Techna Institute for the Advancement of Technology for Health, University Health Network, 100 College Street, Toronto, Ontario M5G 1P5, Canada.
Anal Chem ; 93(10): 4408-4416, 2021 03 16.
Article em En | MEDLINE | ID: mdl-33651938
Spatially resolved ambient mass spectrometry imaging methods have gained popularity to characterize cancer sites and their borders using molecular changes in the lipidome. This utility, however, is predicated on metabolic homogeneity at the border, which would create a sharp molecular transition at the morphometric borders. We subjected murine models of human medulloblastoma brain cancer to mass spectrometry imaging, a technique that provides a direct readout of tissue molecular content in a spatially resolved manner. We discovered a distance-dependent gradient of cancer-like lipid molecule profiles in the brain tissue within 1.2 mm of the cancer border, suggesting that a cancer-like state progresses beyond the histologic border, into the healthy tissue. The results were further corroborated using orthogonal liquid chromatography and mass spectrometry (LC-MS) analysis of selected tissue regions subjected to laser capture microdissection. LC-MS/MS analysis for robust identification of the affected molecules implied changes in a number of different lipid classes, some of which are metabolized from the essential docosahexaenoic fatty acid (DHA) present in the interstitial fluid. Metabolic molecular borders are thus not as sharp as morphometric borders, and mass spectrometry imaging can reveal molecular nuances not observed with microscopy. Caution must be exercised in interpreting multimodal imaging results stipulated on a coincidental relationship between metabolic and morphometric borders of cancer, at least within animal models used in preclinical research.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espectrometria de Massas em Tandem / Neoplasias Limite: Animals / Humans Idioma: En Revista: Anal Chem Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Canadá

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espectrometria de Massas em Tandem / Neoplasias Limite: Animals / Humans Idioma: En Revista: Anal Chem Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Canadá