Production and purification of Clostridium perfringens type D epsilon toxin and IgY antitoxin.
Anaerobe
; 69: 102354, 2021 Jun.
Article
em En
| MEDLINE
| ID: mdl-33675994
ABSTRACT
The aim of this study was to purify Clostridium perfringens type D epsilon toxin and produce and purify anti-epsilon chicken immunoglobulin Y (IgY). A single-step ion exchange chromatography resulted in a high-yield and high-purity toxin, while ion exchange chromatography followed by gel filtration resulted in the highest purity of the toxin, but at a lower yield. Purified and inactivated epsilon toxin were then administered in chickens via four inoculations and IgY was obtained at a high purity and yield, with an antibody titer of 50 IU/mL and high levels of avidity (73.2%). In summary, C. perfringens type D epsilon toxin and chicken anti-epsilon IgY were successfully produced and purified, and may be used for the diagnosis of enterotoxemia caused by the epsilon toxin, as well as in potency tests of existing and future vaccines against enterotoxemia.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Toxinas Bacterianas
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Imunoglobulinas
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Galinhas
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Clostridium perfringens
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Enterotoxemia
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Anticorpos Antibacterianos
Limite:
Animals
Idioma:
En
Revista:
Anaerobe
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Brasil