Heparin-mediated antibiotic delivery from an electrochemically-aligned collagen sheet.
Biomed Mater Eng
; 32(3): 159-170, 2021.
Article
em En
| MEDLINE
| ID: mdl-33780355
ABSTRACT
BACKGROUND:
Implantable medical devices and hardware are prolific in medicine, but hardware associated infections remain a major issue.OBJECTIVE:
To develop and evaluate a novel, biologic antimicrobial coating for medical implants.METHODS:
Electrochemically compacted collagen sheets with and without crosslinked heparin were synthesized per a protocol developed by our group. Sheets were incubated in antibiotic solution (gentamicin or moxifloxacin) overnight, and in vitro activity was assessed with five-day diffusion assays against Pseudomonas aeruginosa. Antibiotic release over time from gentamicin-infused sheets was determined using in vitro elution and high performance liquid chromatography (HPLC).RESULTS:
Collagen-heparin-antibiotic sheets demonstrated larger growth inhibition zones against P. aeruginosa compared to collagen-antibiotic alone sheets. This activity persisted for five days and was not impacted by rinsing sheets prior to evaluation. Rinsed collagen-antibiotic sheets did not produce any inhibition zones. Elution of gentamicin from collagen-heparin-gentamicin sheets was gradual and remained above the minimal inhibitory concentration for gentamicin-sensitive organisms for 29 days. Conversely, collagen-gentamicin sheets eluted their antibiotic load within 24 hours. Overall, heparin-associated sheets demonstrated larger inhibition zones against P. aeruginosa and prolonged elution profile via HPLC.CONCLUSION:
We developed a novel, local antibiotic delivery system that could be used to coat medical implants/hardware in the future and reduce post-operative infections.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Heparina
Idioma:
En
Revista:
Biomed Mater Eng
Assunto da revista:
BIOTECNOLOGIA
/
ENGENHARIA BIOMEDICA
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Estados Unidos