Stat5B is required for IgE-Mediated mast cell function in vitro and in vivo.
Cell Immunol
; 364: 104344, 2021 06.
Article
em En
| MEDLINE
| ID: mdl-33780747
ABSTRACT
Mast cells are found primarily at interfaces with the external environment, where they provide protection from pathogens but also elicit allergic inflammation. Mast cell activation by antigen-induced aggregation of IgE bound to the high affinity receptor, FcεRI, is a critical factor leading to inflammation and bronchoconstriction. We previously found that Stat5 is activated by FcεRI and that Stat5B suppression decreased IgE-induced cytokine production in vitro, but in vivo responses have not been assessed. We now show that Stat5B-deficient (KO) mice have reduced responses to IgE-mediated anaphylaxis, despite normal mast cell tissue distribution. Similarly, Stat5B KO mast cells have diminished IgE-induced degranulation and cytokine secretion in vitro. These mice have elevated IgE production that is not correlated with an intrinsic B cell defect. The current work demonstrates that the Stat5B isoform is required for normal mast cell function and suggests it limits IgE production in vivo.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Imunoglobulina E
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Linfócitos B
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Receptores de IgE
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Fator de Transcrição STAT5
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Hipersensibilidade
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Anafilaxia
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Mastócitos
Limite:
Animals
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Humans
Idioma:
En
Revista:
Cell Immunol
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Estados Unidos