[Chronic toxicity of Multi-walled carbon nanotubes in human pleural mesothelial cells].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
; 39(3): 173-177, 2021 Mar 20.
Article
em Zh
| MEDLINE
| ID: mdl-33781031
ABSTRACT
Objective:
To explore the chronic toxicity and its potential mechanism of multi-walled carbon nanotube (MWCNT) in human pleural mesothelial cells.Methods:
A sustainable exposure of MeT-5A cells to MWCNT at 10 µg/cm(2) for one year was conducted in 2016. During the exposure, the cell images and cell proliferation was recorded every 4 weeks. The cell apoptosis, cell cycle, cell migration and cell invasion were compared between the control cells and the cells after MWCNT exposure. Finally, the gene expression was screened with Affymetrix clariom D assay, and some of the significantly differential expressed genes was verified by RT-PCR.Results:
Compared with the control group, the proliferation ability of the cells in the 1-year exposed group was significantly increased, and the rate of proliferation was about 2-3 times as that in the Control Group (F=481.32, P<0.05) . MeT-5A cells all showed cell cycle arrest effect, which showed the increase of G1 phase and the decrease of s phase and G2 phase (F=14.94, P<0.05) . The apoptosis rate of cells in the treated group was significantly higher than that in the control group after 6 months (F=15.12, P<0.05) , but the early apoptosis rate and the total apoptosis rate of cells in the treated group were not significantly different from those in the control group after 1 year (F=3.97, P<0.05) . The cell migration and invasion were both promoted by MWCNT. Furthermore, the differentially expressed genes was screened, to find 2, 878 genes with more than 2 folds changes. To further verified, RT-PCR was conducted with PIK3R3ãWNT2BãVANGL2ãANXA1, and their expression changes were consistent with above.Conclusion:
MWCNT might have a carcinogenic potential to MeT-5A cells after the long term exposure.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Nanotubos de Carbono
Limite:
Humans
Idioma:
Zh
Revista:
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
Assunto da revista:
MEDICINA OCUPACIONAL
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China