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Molecular-scale axial localization by repetitive optical selective exposure.
Gu, Lusheng; Li, Yuanyuan; Zhang, Shuwen; Zhou, Maoge; Xue, Yanhong; Li, Weixing; Xu, Tao; Ji, Wei.
Afiliação
  • Gu L; National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
  • Li Y; Physical Science Laboratory, Huairou National Comprehensive Science Center, Beijing, China.
  • Zhang S; Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangzhou, Guangdong, China.
  • Zhou M; College of Life Science, University of Chinese Academy of Sciences, Beijing, China.
  • Xue Y; Core Facility for Protein Research, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
  • Li W; National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
  • Xu T; Physical Science Laboratory, Huairou National Comprehensive Science Center, Beijing, China.
  • Ji W; Core Facility for Protein Research, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
Nat Methods ; 18(4): 369-373, 2021 04.
Article em En | MEDLINE | ID: mdl-33795876
We introduce an axial localization with repetitive optical selective exposure (ROSE-Z) method for super-resolution imaging. By using an asymmetric optical scheme to generate interference fringes, a <2 nm axial localization precision was achieved with only ~3,000 photons, which is an approximately sixfold improvement compared to previous astigmatism methods. Nanoscale three-dimensional and two-color imaging was demonstrated, illustrating how this method achieves superior performance and facilitates the investigation of cellular nanostructures.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Imageamento Tridimensional / Microscopia Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Imageamento Tridimensional / Microscopia Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China