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Electrochemical Immunosensor for the Quantification of S100B at Clinically Relevant Levels Using a Cysteamine Modified Surface.
Rodríguez, Alexander; Burgos-Flórez, Francisco; Posada, José D; Cervera, Eliana; Zucolotto, Valtencir; Sanjuán, Homero; Sanjuán, Marco; Villalba, Pedro J.
Afiliação
  • Rodríguez A; Biotechnology Research Group, Universidad del Norte, Barranquilla 081007, Colombia.
  • Burgos-Flórez F; Biotechnology Research Group, Universidad del Norte, Barranquilla 081007, Colombia.
  • Posada JD; Rational Use of Energy and Preservation of the Environment Group (UREMA), Universidad del Norte, Barranquilla 081007, Colombia.
  • Cervera E; School of Medicine, Stanford University, Redwood City, CA 94063, USA.
  • Zucolotto V; Biotechnology Research Group, Universidad del Norte, Barranquilla 081007, Colombia.
  • Sanjuán H; Gnano-Nanomedicine and Nanotoxicology Group, São Carlos Institute of Physics, University of São Paulo, São Carlos 13566-590, São Paulo, Brazil.
  • Sanjuán M; Biotechnology Research Group, Universidad del Norte, Barranquilla 081007, Colombia.
  • Villalba PJ; Rational Use of Energy and Preservation of the Environment Group (UREMA), Universidad del Norte, Barranquilla 081007, Colombia.
Sensors (Basel) ; 21(6)2021 Mar 10.
Article em En | MEDLINE | ID: mdl-33801798
ABSTRACT
Neuronal damage secondary to traumatic brain injury (TBI) is a rapidly evolving condition, which requires therapeutic decisions based on the timely identification of clinical deterioration. Changes in S100B biomarker levels are associated with TBI severity and patient outcome. The S100B quantification is often difficult since standard immunoassays are time-consuming, costly, and require extensive expertise. A zero-length cross-linking approach on a cysteamine self-assembled monolayer (SAM) was performed to immobilize anti-S100B monoclonal antibodies onto both planar (AuEs) and interdigitated (AuIDEs) gold electrodes via carbonyl-bond. Surface characterization was performed by atomic force microscopy (AFM) and specular-reflectance FTIR for each functionalization step. Biosensor response was studied using the change in charge-transfer resistance (Rct) from electrochemical impedance spectroscopy (EIS) in potassium ferrocyanide, with [S100B] ranging 10-1000 pg/mL. A single-frequency analysis for capacitances was also performed in AuIDEs. Full factorial designs were applied to assess biosensor sensitivity, specificity, and limit-of-detection (LOD). Higher Rct values were found with increased S100B concentration in both platforms. LODs were 18 pg/mL(AuES) and 6 pg/mL(AuIDEs). AuIDEs provide a simpler manufacturing protocol, with reduced fabrication time and possibly costs, simpler electrochemical response analysis, and could be used for single-frequency analysis for monitoring capacitance changes related to S100B levels.
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Texto completo: 1 Base de dados: MEDLINE Tipo de estudo: Guideline / Prognostic_studies Idioma: En Revista: Sensors (Basel) Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Colômbia

Texto completo: 1 Base de dados: MEDLINE Tipo de estudo: Guideline / Prognostic_studies Idioma: En Revista: Sensors (Basel) Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Colômbia