The Ins and Outs of Messenger RNA Electroporation for Physical Gene Delivery in Immune Cell-Based Therapy.
Pharmaceutics
; 13(3)2021 Mar 16.
Article
em En
| MEDLINE
| ID: mdl-33809779
ABSTRACT
Messenger RNA (mRNA) electroporation is a powerful tool for transient genetic modification of cells. This non-viral method of genetic engineering has been widely used in immunotherapy. Electroporation allows fine-tuning of transfection protocols for each cell type as well as introduction of multiple protein-coding mRNAs at once. As a pioneering group in mRNA electroporation, in this review, we provide an expert overview of the ins and outs of mRNA electroporation, discussing the different parameters involved in mRNA electroporation as well as the production of research-grade and production and application of clinical-grade mRNA for gene transfer in the context of cell-based immunotherapies.
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Base de dados:
MEDLINE
Tipo de estudo:
Guideline
Idioma:
En
Revista:
Pharmaceutics
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Bélgica