A novel chondroitin sulfate E from Dosidicus gigas cartilage and its antitumor metastatic activity.
Carbohydr Polym
; 262: 117971, 2021 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-33838835
ABSTRACT
Chondroitin sulfate (CS) chains containing GlcUAß1-3GalNAc(4S,6S) (E unit) have been shown to be involved in various physiological and pathological processes. However, commercial E unit-rich CS (CS-E) is difficult to produce on a large scale due to expensive and limited squid cartilage resources. In this study, a novel CS-E (CS-nE) was isolated from the cheap and abundant cartilage of the giant squid Dosidicus gigas. The CS-nE has a surprisingly large molecular mass of 696 kDa and a relatively high E unit proportion (44.5 %). It can interact with various growth factors, including HGF, bFGF, pleiotrophin, and HB-EGF, with high affinity, and exhibits dose-dependent anti-metastatic activity. Furthermore, the E unit-rich decasaccharide selectively prepared from CS-nE has been shown to be the minimal functional domain with the strongest antitumor metastatic activity. Taken together, CS-nE will be a very promising candidate for the development of CS-E-based pharmaceutical products.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Decapodiformes
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Cartilagem
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Sulfatos de Condroitina
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Antineoplásicos
Limite:
Animals
Idioma:
En
Revista:
Carbohydr Polym
Ano de publicação:
2021
Tipo de documento:
Article