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Changes in the phosphorylation of nucleotide metabolism­associated proteins by leukemia inhibitory factor in mouse embryonic stem cells.
Song, Hwa-Ryung; Kim, Han-Kyu; Kim, Seung-Gook; Lim, Hyung-Jin; Kim, Hyun-Yi; Han, Myung-Kwan.
Afiliação
  • Song HR; Department of Microbiology, Jeonbuk National University Medical School, Jeonju, Jeollabuk 54896, Republic of Korea.
  • Kim HK; Department of Microbiology, Jeonbuk National University Medical School, Jeonju, Jeollabuk 54896, Republic of Korea.
  • Kim SG; Department of Microbiology, Jeonbuk National University Medical School, Jeonju, Jeollabuk 54896, Republic of Korea.
  • Lim HJ; Department of Microbiology, Jeonbuk National University Medical School, Jeonju, Jeollabuk 54896, Republic of Korea.
  • Kim HY; Division of Anatomy and Developmental Biology, Department of Oral Biology, Yonsei University College of Dentistry, Seoul 03722, Republic of Korea.
  • Han MK; Department of Microbiology, Jeonbuk National University Medical School, Jeonju, Jeollabuk 54896, Republic of Korea.
Mol Med Rep ; 23(6)2021 06.
Article em En | MEDLINE | ID: mdl-33846773
Leukemia inhibitory factor (LIF) is a stem cell growth factor that maintains self­renewal of mouse embryonic stem cells (mESCs). LIF is a cytokine in the interleukin­6 family and signals via the common receptor subunit gp130 and ligand­specific LIF receptor. LIF causes heterodimerization of the LIF receptor and gp130, activating the Janus kinase/STAT and MAPK pathways, resulting in changes in protein phosphorylation. The present study profiled LIF­mediated protein phosphorylation changes in mESCs via proteomic analysis. mESCs treated in the presence or absence of LIF were analyzed via two­dimensional differential in­gel electrophoresis and protein and phosphoprotein staining. Protein identification was performed by matrix­assisted laser desorption/ionization­time of flight mass spectrophotometry. Increased phosphorylation of 16 proteins and decreased phosphorylation of 34 proteins in response to LIF treatment was detected. Gene Ontology terms enriched in these proteins included 'organonitrogen compound metabolic process', 'regulation of mRNA splicing via spliceosome' and 'nucleotide metabolic process'. The present results revealed that LIF modulated phosphorylation levels of nucleotide metabolism­associated proteins, thus providing insight into the mechanism underlying LIF action in mESCs.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fator Inibidor de Leucemia / Células-Tronco Embrionárias Murinas / Nucleotídeos Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals Idioma: En Revista: Mol Med Rep Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fator Inibidor de Leucemia / Células-Tronco Embrionárias Murinas / Nucleotídeos Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals Idioma: En Revista: Mol Med Rep Ano de publicação: 2021 Tipo de documento: Article