Activated monocytes induce arthritis-associated changes in mitochondria of cultured synovial fibroblasts.
Scand J Rheumatol
; 17(2): 131-41, 1988.
Article
em En
| MEDLINE
| ID: mdl-3387930
ABSTRACT
We have recently shown that synovial fibroblasts cultured from patients with reactive or rheumatoid arthritis exhibit increased autofluorescence when compared with controls. Morphological studies suggested that this increase was related to the anomalous structure of mitochondria in cells cultured from rheumatoid or non-rheumatoid inflammatory synovial tissue. The present study describes attempts to find an explanation for these observations. The effects of conditioned media of cultured mononuclear cells were tested on normal synovial fibroblasts. Conditioned media of monocytes stimulated with lipopolysaccharide or poly-IC induced an increase in the cellular autofluorescence and changes in the morphology of mitochondria in normal fibroblasts. These changes were indistinguishable from those seen in synovial fibroblasts cultured from various arthritides. Indomethacin or gold salts did not abolish the effects of monocyte-conditioned media. Abnormal mitochondria could not be induced in the presence of cycloheximide. This study describes a new aspect of monocyte-fibroblast interactions during rheumatoid and non-rheumatoid inflammation of synovial tissue.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Artrite Reumatoide
/
Líquido Sinovial
/
Ativação de Macrófagos
/
Mitocôndrias
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Revista:
Scand J Rheumatol
Ano de publicação:
1988
Tipo de documento:
Article
País de afiliação:
Finlândia