Optimized cryo-EM data-acquisition workflow by sample-thickness determination.

Rheinberger, Jan; Oostergetel, Gert; Resch, Guenter P; Paulino, Cristina

Rheinberger, Jan; Oostergetel, Gert; Resch, Guenter P; Paulino, Cristina.

Afiliação

Rheinberger J; Department of Structural Biology and Membrane Enzymology at the Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands.
Oostergetel G; Department of Structural Biology and Membrane Enzymology at the Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands.
Resch GP; Nexperion e.U. - Solutions for Electron Microscopy, Vienna, Austria.
Paulino C; Department of Structural Biology and Membrane Enzymology at the Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands.

Acta Crystallogr D Struct Biol ; 77(Pt 5): 565-571, 2021 May 01.

Article em En | MEDLINE | ID: mdl-33950013

Assuntos

Microscopia Crioeletrônica/métodos; Frutose-Bifosfato Aldolase/química; Processamento de Imagem Assistida por Computador/métodos; Manejo de Espécimes/métodos; Animais; Coelhos; Software

Palavras-chave

Digital Micrograph; SerialEM; automation; sample thickness; single-particle cryo-electron microscopy

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Manejo de Espécimes / Processamento de Imagem Assistida por Computador / Microscopia Crioeletrônica / Frutose-Bifosfato Aldolase Limite: Animals Idioma: En Revista: Acta Crystallogr D Struct Biol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Holanda

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