Reduced A-to-I editing of endogenous Alu RNAs in lung after SARS-CoV-2 infection.
Curr Res Immunol
; 2: 52-59, 2021.
Article
em En
| MEDLINE
| ID: mdl-33969287
ABSTRACT
Due to potential severity of disease caused by SARS-CoV-2 infection, it is critical to understand both mechanisms of viral pathogenesis as well as diversity of host responses to infection. Reduced A-to-I editing of endogenous double-stranded RNAs (dsRNAs), as a result of inactivating mutations in ADAR, produces one form of Aicardi-Goutières Syndrome, with an immune response similar to an anti-viral response. By analyzing whole genome RNA sequencing data, we find reduced levels of A-to-I editing of endogenous Alu RNAs in normal human lung cells after infection by SARS-CoV-2 as well as in lung biopsies from patients with SARS-CoV-2 infections. Unedited Alu RNAs, as seen after infection, induce IRF and NF-kB transcriptional responses and downstream target genes, while edited Alu RNAs as seen in the absence of infection, fail to activate these transcriptional responses. Thus, decreased A-to-I editing may represent an important host response to SARS-CoV-2 infection.
A-to-I editing; A-to-I, adenosine-to-inosine; Alu RNAs; Double-stranded RNA sensors; EEL, editing enriched location; IRs, inverted repeats; ISGs, interferon-stimulated genes; NHBE, normal human bronchial epithelial cells; RNA-seq, whole genome RNA-sequencing; SARS-CoV-2; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2
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Base de dados:
MEDLINE
Idioma:
En
Revista:
Curr Res Immunol
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Estados Unidos