Progress toward Developing Sensitive Non-Sputum-Based Tuberculosis Diagnostic Tests: the Promise of Urine Cell-Free DNA.
J Clin Microbiol
; 59(8): e0070621, 2021 07 19.
Article
em En
| MEDLINE
| ID: mdl-33980646
ABSTRACT
A highly accurate, non-sputum-based test for tuberculosis (TB) detection is a key priority for the field of TB diagnostics. A recent study in the Journal of Clinical Microbiology by Oreskovic and colleagues (J Clin Microbiol 59e00074-21, 2021, https//doi.org/10.1128/JCM.00074-21) reports the performance of an optimized urine cell-free DNA (cfDNA) test using sequence-specific purification combined with short-target PCR to improve the accuracy of TB detection. Their retrospective clinical study utilized frozen urine samples (n = 73) from study participants diagnosed with active pulmonary TB in South Africa and compared results to non-TB patients in South Africa and the United States in an early-phase validation study. Overall, this cfDNA technique detected TB with a sensitivity of 83.7% (95% CI 71.0 to 91.5) and specificity of 100% (95% CI 86.2 to 100), which meet the World Health Organization's published performance criteria. Sensitivity was 73.3% in people without HIV (95% CI 48.1 to 89.1) and 76% in people with smear-negative TB (95% CI 56.5 to 88.5). In this commentary, we discuss the results of this optimized urine TB cfDNA assay within the larger context of TB diagnostics and pose additional questions for further research.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Tuberculose
/
Ácidos Nucleicos Livres
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
Limite:
Humans
País/Região como assunto:
Africa
Idioma:
En
Revista:
J Clin Microbiol
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Canadá