Cloning of a lymphoid-specific cDNA encoding a protein binding the regulatory octamer DNA motif.
Science
; 241(4865): 577-80, 1988 Jul 29.
Article
em En
| MEDLINE
| ID: mdl-3399892
ABSTRACT
An octamer DNA sequence plays a critical role in directing transcription of immunoglobulin genes in B lymphocytes. A new technique of direct binding of radioactive DNA was used to screen a complementary DNA expression library from the BJAB cell line in lambda gt11 phage to derive molecular cDNA clones representing a putative B lymphocyte-specific octamer binding protein. The plaques were screened with DNA containing four copies of the octamer sequence and positive phage recombinants were identified. The fusion protein produced on inducing a lysogen of one phage bound to a monomeric octamer probe. The cDNA insert from this phage hybridized to messenger RNA found in B lymphocytes, but not in most other cells. Thus, this cDNA derives from a gene (oct-2) that specifies an octamer binding protein expressed preferentially in B lymphocytes, proving that, for at least one gene, a cell-specific transcription factor exists and its amount is controlled through messenger RNA availability.
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Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Linfócitos
/
Sequências Reguladoras de Ácido Nucleico
/
Proteínas de Ligação a DNA
/
Genes
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Science
Ano de publicação:
1988
Tipo de documento:
Article