Preparation and evaluation of a polymer-based sulfobetaine zwitterionic stationary phase.
J Chromatogr A
; 1649: 462229, 2021 Jul 19.
Article
em En
| MEDLINE
| ID: mdl-34038779
ABSTRACT
We describe a polymer-based sulfobetaine zwitterionic stationary phase for hydrophilic interaction liquid chromatography (HILIC). It was prepared by grafting acrylamide-type sulfobetaine monomer instead of common methacrylate-type sulfobetaine onto hydrolysed poly (glycidyl methacrylate-divinylbenzene) (GMA-DVB) microspheres via pendant double bonds of DVB. The phase has been characterized by elemental analysis, scanning electron micrograph and N2 adsorption-desorption experiment. It shows wider pH tolerance range (from 2 to 12) and excellent separation ability towards common strong polar analytes such as nucleosides and nucleic bases, water-soluble vitamins, amino acids, inorganic anions and cations. Notably, it exhibits negligible baseline noise level (~0.15 pA) under typical HILIC mobile phase. Excellent selectivity in separation of α- and ß-anomers of reducing sugars and lactose/lactulose has also been observed.
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Base de dados:
MEDLINE
Assunto principal:
Polímeros
/
Betaína
/
Cromatografia Líquida
Idioma:
En
Revista:
J Chromatogr A
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China