Early maternal deprivation impairs learning and memory and alters hippocampal gene expression in adult male rats.
Neurobiol Learn Mem
; 183: 107479, 2021 09.
Article
em En
| MEDLINE
| ID: mdl-34119613
ABSTRACT
Maternal deprivation (MD) in early life severely disrupts hippocampal development, leading to persistent cognitive and behavior deficits. The current study uncovered that early MD (P1-P21) impaired spatial learning and memory capacity detected by Morris water maze (MWM) tests from juvenile (P31) to adult (P81) rats compared to age-matched controls. And the protein expression in hippocampus were detected by two-dimensional gel electrophoresis (2-DE) before MWM, respectively. Protein changes in hippocampal were examined to identify the molecular pathways underlying MD-induced hippocampal dysfunction. There were 11 differentially expressed proteins analyzed between adult MD and control male rats, while the 8 proteins were then identified by UPLC-ESI-Q-TOF-MS. Gene Ontology (GO) annotations of the identified proteins were related to neuronal and glial cytoskeletal dynamics, membrane signaling, stress responses, biosynthesis, and metabolism. The different expression proteins spectrin alpha chain, non-erythrocytic 1 (Sptan1), ATP-citrate synthase (Acly), and heat shock protein 90-alpha (Hsp90aa1) have been verified by western blot analysis, and their expression levels showed consistent with 2-DE analysis. In addition, glial fibrillary acidic protein (GFAP) was also found reduced in adult hippocampus of MD rats. This study identifies candidate proteins encompassing a range of functional categories that may contribute to persistent learning and memory deficits due to early life MD.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
ATP Citrato (pro-S)-Liase
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Proteínas de Choque Térmico HSP90
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Proteínas de Transporte Vesicular
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Aprendizagem Espacial
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Memória Espacial
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Proteína Glial Fibrilar Ácida
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Hipocampo
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Privação Materna
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Proteínas dos Microfilamentos
Limite:
Animals
Idioma:
En
Revista:
Neurobiol Learn Mem
Assunto da revista:
BIOLOGIA
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CIENCIAS DO COMPORTAMENTO
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NEUROLOGIA
Ano de publicação:
2021
Tipo de documento:
Article