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Electrospun core-shell nanofibers with encapsulated enamel matrix derivative for guided periodontal tissue regeneration.
Lam, Linda R Wang; Schilling, Kevin; Romas, Stephen; Misra, Ravi; Zhou, Zhuang; Caton, Jack G; Zhang, Xinping.
Afiliação
  • Lam LRW; Center for Musculoskeletal Research, University of Rochester, School of Medicine and Dentistry.
  • Schilling K; Department of Periodontology, Eastman Institute for Oral Health, University of Rochester, School of Medicine and Dentistry.
  • Romas S; Center for Musculoskeletal Research, University of Rochester, School of Medicine and Dentistry.
  • Misra R; Department of Biomedical Engineering, University of Rochester.
  • Zhou Z; Department of Pediatrics, University of Rochester, School of Medicine and Dentistry.
  • Caton JG; Department of Pediatrics, University of Rochester, School of Medicine and Dentistry.
  • Zhang X; Center for Musculoskeletal Research, University of Rochester, School of Medicine and Dentistry.
Dent Mater J ; 40(5): 1208-1216, 2021 Sep 30.
Article em En | MEDLINE | ID: mdl-34121026
The osteogenic effect of a composite electrospun core-shell nanofiber membrane encapsulated with Emdogain® (EMD) was evaluated. The membrane was developed through coaxial electrospinning using polycaprolactone as the shell and polyethylene glycol as the core. The effects of the membrane on the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) were examined using Alizarin Red S staining and qRT-PCR. Characterization of the nanofiber membrane demonstrated core-shell morphology with a mean diameter of ~1 µm. Examination of the release of fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) from core-shell nanofibers over a 22-day period showed improved release profile of encapsulated proteins as compared to solid nanofibers. When cultured on EMD-containing core-shell nanofibers, PDLSCs showed significantly improved osteogenic differentiation with increased Alizarin Red S staining and enhanced osteogenic gene expression, namely OCN, RUNX2, ALP, and OPN. Core-shell nanofiber membranes may improve outcomes in periodontal regenerative therapy through simultaneous mechanical barrier and controlled drug delivery function.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Nanofibras Idioma: En Revista: Dent Mater J Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Nanofibras Idioma: En Revista: Dent Mater J Ano de publicação: 2021 Tipo de documento: Article