Spinning pupil aberration measurement for anisoplanatic deconvolution.
Opt Lett
; 46(12): 2884-2887, 2021 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-34129565
ABSTRACT
The aberrations in an optical microscope are commonly measured and corrected at one location in the field of view, within the so-called isoplanatic patch. Full-field correction is desirable for high-resolution imaging of large specimens. Here we present, to the best of our knowledge, a novel wavefront detector, based on pupil sampling with subapertures, measuring the aberrated wavefront phase at each position of the specimen. Based on this measurement, we propose a region-wise deconvolution that provides an anisoplanatic reconstruction of the sample image. Our results indicate that the measurement and correction of the aberrations can be performed in a wide-field fluorescence microscope over its entire field of view.
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MEDLINE
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En
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Opt Lett
Ano de publicação:
2021
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Article