Protocol to establish cancer-associated fibroblasts from surgically resected tissues and generate senescent fibroblasts.

Yasuda, Tadahito; Koiwa, Mayu; Yonemura, Atsuko; Akiyama, Takahiko; Baba, Hideo; Ishimoto, Takatsugu

Yasuda, Tadahito; Koiwa, Mayu; Yonemura, Atsuko; Akiyama, Takahiko; Baba, Hideo; Ishimoto, Takatsugu.

Afiliação

Yasuda T; Department of Gastroenterological Surgery, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan.
Koiwa M; Gastrointestinal Cancer Biology, International Research Center for Medical Sciences (IRCMS), Kumamoto University, Kumamoto, Japan.
Yonemura A; Department of Gastroenterological Surgery, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan.
Akiyama T; Gastrointestinal Cancer Biology, International Research Center for Medical Sciences (IRCMS), Kumamoto University, Kumamoto, Japan.
Baba H; Department of Gastroenterological Surgery, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan.
Ishimoto T; Gastrointestinal Cancer Biology, International Research Center for Medical Sciences (IRCMS), Kumamoto University, Kumamoto, Japan.

STAR Protoc ; 2(2): 100553, 2021 06 18.

Article em En | MEDLINE | ID: mdl-34136831

Assuntos

Fibroblastos Associados a Câncer/citologia; Senescência Celular; Neoplasias Gástricas/cirurgia; Fibroblastos Associados a Câncer/metabolismo; Citocinas/metabolismo; Humanos; Mediadores da Inflamação/metabolismo; Neoplasias Gástricas/metabolismo; Microambiente Tumoral

Palavras-chave

Cancer; Cell Biology; Cell culture; Cell isolation

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias Gástricas / Senescência Celular / Fibroblastos Associados a Câncer Tipo de estudo: Risk_factors_studies Limite: Humans Idioma: En Revista: STAR Protoc Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Japão

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