MiR-99b-5p suppressed proliferation of human osteoblasts by targeting FGFR3 in osteoporosis.
Hum Cell
; 34(5): 1398-1409, 2021 Sep.
Article
em En
| MEDLINE
| ID: mdl-34156640
ABSTRACT
Osteoporosis is a common skeletal disease characterized by reduced bone mass partially caused by an imbalance between bone resorption and formation. Considering the potential role of microRNAs (miRNAs) in osteoporosis, we attempted to identify deregulated miRNA that participates in the pathogenesis of osteoporosis. We analyzed online datasets for differentially expressed miRNAs and predicted deregulated miRNA target genes, applied these genes for signaling pathway enrichment annotation, and selected the possible miR-99b-5p/FGFR3 axis. Within osteoporosis bone tissues, miR-99b-5p was upregulated and FGFR3 was downregulated. miR-99b-5p overexpression inhibited osteoblast proliferation and osteogenesis-related genes expression, whereas FGFR3 overexpression exerted opposite effects upon the proliferation of osteoblasts and osteogenesis-related genes expression. By direct targeting, miR-99b-5p inhibited FGFR3 expression. Moreover, FGFR3 silencing significantly reversed the roles of miR-99b-5p inhibition in the proliferation of osteoblasts and osteogenesis-related genes expression. In conclusion, we identify a deregulated miRNA/mRNA axis in osteoporosis and osteogenic differentiation, namely the miR-99b-5p/FGFR3 axis; through targeting FGFR3, miR-99b-5p inhibits osteoblast proliferation and activity, which might subsequently affect the bone formation in osteoporosis progression.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Osteoblastos
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Osteoporose
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Regulação da Expressão Gênica no Desenvolvimento
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MicroRNAs
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Proliferação de Células
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Receptor Tipo 3 de Fator de Crescimento de Fibroblastos
Tipo de estudo:
Prognostic_studies
Limite:
Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Hum Cell
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China