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SUMO fosters assembly and functionality of the MutSγ complex to facilitate meiotic crossing over.
He, Wei; Verhees, Gerrik F; Bhagwat, Nikhil; Yang, Ye; Kulkarni, Dhananjaya S; Lombardo, Zane; Lahiri, Sudipta; Roy, Pritha; Zhuo, Jiaming; Dang, Brian; Snyder, Andriana; Shastry, Shashank; Moezpoor, Michael; Alocozy, Lilly; Lee, Kathy Gyehyun; Painter, Daniel; Mukerji, Ishita; Hunter, Neil.
Afiliação
  • He W; Howard Hughes Medical Institute, University of California, Davis, Davis, CA, USA; Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, CA, USA.
  • Verhees GF; Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, CA, USA.
  • Bhagwat N; Howard Hughes Medical Institute, University of California, Davis, Davis, CA, USA; Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, CA, USA.
  • Yang Y; Howard Hughes Medical Institute, University of California, Davis, Davis, CA, USA; Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, CA, USA.
  • Kulkarni DS; Howard Hughes Medical Institute, University of California, Davis, Davis, CA, USA; Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, CA, USA.
  • Lombardo Z; Department of Molecular Biology and Biochemistry, Molecular Biophysics Program, Wesleyan University, Middletown, CT, USA.
  • Lahiri S; Department of Molecular Biology and Biochemistry, Molecular Biophysics Program, Wesleyan University, Middletown, CT, USA.
  • Roy P; Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, CA, USA.
  • Zhuo J; Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, CA, USA.
  • Dang B; Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, CA, USA.
  • Snyder A; Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, CA, USA.
  • Shastry S; Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, CA, USA.
  • Moezpoor M; Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, CA, USA.
  • Alocozy L; Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, CA, USA.
  • Lee KG; Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, CA, USA.
  • Painter D; Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, CA, USA.
  • Mukerji I; Department of Molecular Biology and Biochemistry, Molecular Biophysics Program, Wesleyan University, Middletown, CT, USA.
  • Hunter N; Howard Hughes Medical Institute, University of California, Davis, Davis, CA, USA; Department of Microbiology & Molecular Genetics, University of California, Davis, Davis, CA, USA. Electronic address: nhunter@ucdavis.edu.
Dev Cell ; 56(14): 2073-2088.e3, 2021 07 26.
Article em En | MEDLINE | ID: mdl-34214491
ABSTRACT
Crossing over is essential for chromosome segregation during meiosis. Protein modification by SUMO is implicated in crossover control, but pertinent targets have remained elusive. Here we identify Msh4 as a target of SUMO-mediated crossover regulation. Msh4 and Msh5 constitute the MutSγ complex, which stabilizes joint-molecule (JM) recombination intermediates and facilitates their resolution into crossovers. Msh4 SUMOylation enhances these processes to ensure that each chromosome pair acquires at least one crossover. Msh4 is directly targeted by E2 conjugase Ubc9, initially becoming mono-SUMOylated in response to DNA double-strand breaks, then multi/poly-SUMOylated forms arise as homologs fully engage. Mechanistically, SUMOylation fosters interaction between Msh4 and Msh5. We infer that initial SUMOylation of Msh4 enhances assembly of MutSγ in anticipation of JM formation, while secondary SUMOylation may promote downstream functions. Regulation of Msh4 by SUMO is distinct and independent of its previously described stabilization by phosphorylation, defining MutSγ as a hub for crossover control.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Troca Genética / Proteínas de Saccharomyces cerevisiae / Proteínas Modificadoras Pequenas Relacionadas à Ubiquitina / Proteínas de Ligação a DNA / Sumoilação / Meiose Idioma: En Revista: Dev Cell Assunto da revista: EMBRIOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Troca Genética / Proteínas de Saccharomyces cerevisiae / Proteínas Modificadoras Pequenas Relacionadas à Ubiquitina / Proteínas de Ligação a DNA / Sumoilação / Meiose Idioma: En Revista: Dev Cell Assunto da revista: EMBRIOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos