MiR-191-5p is upregulated in culture media of implanted human embryo on day fifth of development.
Reprod Biol Endocrinol
; 19(1): 109, 2021 Jul 13.
Article
em En
| MEDLINE
| ID: mdl-34256783
ABSTRACT
BACKGROUND:
Morphological features are the most common criteria used to select human embryos for transfer to a receptive uterine cavity. However, such characteristics are not valid for embryos in cellular arrest. Even aneuploid embryos can have normal morphology, and some euploid embryos have aberrant morphology. The aim of this study was to quantify the expression profile of hsa-miR-21-3p, -24-1-5p, -191-5p, and -372-5p in culture media on day 5 of in vitro embryo development, and compare the profiles of two groups of media classified byoutcome:
successful (n = 25) or unsuccessful (n = 25) implantation pregnancy.METHODS:
Fifty patients were accepted in the Department of Reproductive Biology of a Hospital in México City, based on the Institutional inclusion criteria for in vitro fertilization. Embryos were transferred to the women on day 5 of cultivation, and the culture media were collected. RNA was isolated from each culture medium with TRIzol reagent, and microRNA (miRNA) expression was detected through RT-PCR with specific primers. Expression bands were quantified by reading optical density.RESULTS:
There was a 5.2-fold greater expression of hsa-miR-191-5p in the pregnancy-related culture media (p ≤ 0.001) and a 1.6-fold greater level of hsa-miR-24-1-5p (p = 0.043) in the media corresponding to non-pregnant women. No significant difference existed between the two groups hsa-miR-21-3p (p = 0.38) or hsa-miR-372-5p (p = 0.41).CONCLUSIONS:
Regarding adequate in vitro embryo development, hsa-miR-191-5p could possibly represent a positive biomarker, while has-miR-24-1-5p may indicate poor prognosis. This former miRNA modulates IGF2BP-1 and IGF2R, associated with the implantation window. On the other hand, hsa-miR-24-1-5p may be related to a poor prognosis of human embryo development.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Implantação do Embrião
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Regulação para Cima
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Meios de Cultura
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MicroRNAs
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Desenvolvimento Embrionário
Tipo de estudo:
Prognostic_studies
Limite:
Adult
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Female
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Humans
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Pregnancy
Idioma:
En
Revista:
Reprod Biol Endocrinol
Assunto da revista:
ENDOCRINOLOGIA
/
MEDICINA REPRODUTIVA
Ano de publicação:
2021
Tipo de documento:
Article