Association of RDM1 with osteosarcoma progression via cell cycle and MEK/ERK signalling pathway regulation.
J Cell Mol Med
; 25(16): 8039-8046, 2021 08.
Article
em En
| MEDLINE
| ID: mdl-34264012
ABSTRACT
RAD52 motif-containing 1 (RDM1), a key regulator of DNA double-strand break repair and recombination, has been reported to play an important role in the development of various human cancers, such as papillary thyroid carcinoma, neuroblastoma and lung cancer. However, the effect of RDM1 on osteosarcoma (OS) progression remains unclear. Here, this study mainly explored the connection between RDM1 and OS progression, as well as the underlying mechanism. It was found that RDM1 was highly expressed in OS cells compared with human osteoblast cells. Knockdown of RDM1 caused OS cell proliferation inhibition, cell apoptosis promotion and cell cycle arrest at G1 stage, whereas RDM1 overexpression resulted in the opposite phenotypes. Furthermore, RDM1 silencing leads to a significant decrease in tumour growth in xenograft mouse model. RDM1 also increased the protein levels of MEK 1/2 and ERK 1/2. All these findings suggest that RDM1 plays an oncogenic role in OS via stimulating cell cycle transition from G1 to S stage, and regulating MEK/ERK signalling pathway, providing a promising therapeutic factor for the treatment of OS.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Osteossarcoma
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Regulação Neoplásica da Expressão Gênica
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Ciclo Celular
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Sistema de Sinalização das MAP Quinases
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Proteínas de Ligação a DNA
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Reparo do DNA
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Quebras de DNA de Cadeia Dupla
Tipo de estudo:
Prognostic_studies
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Risk_factors_studies
Limite:
Animals
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Female
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Humans
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Male
Idioma:
En
Revista:
J Cell Mol Med
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China