Molecular characterization of mouse CREB3 regulatory factor in Neuro2a cells.
Mol Biol Rep
; 48(7): 5411-5420, 2021 Jul.
Article
em En
| MEDLINE
| ID: mdl-34275032
ABSTRACT
We performed expression and functional analysis of mouse CREB3 regulatory factor (CREBRF) in Neuro2a cells by constructing several expression vectors. Overexpressed full-length (FL) CREBRF protein was stabilized by MG132; however, the intrinsic CREBRF expression in Neuro2a cells was negligible under all conditions. On the other hand, N- or C-terminal deletion of CREBRF influenced its stability. Cotransfection of CREBRF together with GAL4-tagged FL CREB3 increased luciferase reporter activity, and only the N-terminal region of CREBRF was sufficient to potentiate luciferase activity. Furthermore, this positive effect of CREBRF was also observed in cells expressing GAL4-tagged cleaved CREB3, although CREBRF hardly influenced the protein stability of NanoLuc-tagged cleaved CREB3 or intracellular localization of EGFP-tagged one. In conclusion, this study suggests that CREBRF, a quite unstable proteasome substrate, positively regulates the CREB3 pathway, which is distinct from the canonical ER stress pathway in Neuro2a cells.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Ligação a DNA
Limite:
Animals
Idioma:
En
Revista:
Mol Biol Rep
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Japão