ACE2 chromogenic immunostaining protocol optimized for formalin-fixed paraffin-embedded human tissue sections.
STAR Protoc
; 2(3): 100696, 2021 09 17.
Article
em En
| MEDLINE
| ID: mdl-34308375
ABSTRACT
Angiotensin-converting enzyme 2 (ACE2) is a key cellular entry factor for severe acute respiratory syndrome coronavirus 2. Hence, identifying cell types that express ACE2 is important for understanding the pathophysiology of coronavirus disease 2019. We performed extensive testing of multiple primary antibodies across various human tissue types. Here, we describe an optimized protocol for immunostaining of ACE2 in formalin-fixed paraffin-embedded human pancreas, small intestine, and kidney tissue sections obtained from organ donors and autopsies. For complete details on the use and execution of this protocol, please refer to Kusmartseva et al. (2020).
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Imuno-Histoquímica
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Fixação de Tecidos
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Inclusão em Parafina
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Formaldeído
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Enzima de Conversão de Angiotensina 2
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SARS-CoV-2
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COVID-19
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Estados Unidos