Accessing the Cytotoxicity and Cell Response to Biomaterials.
J Vis Exp
; (173)2021 07 08.
Article
em En
| MEDLINE
| ID: mdl-34309590
ABSTRACT
Biomaterials contact directly or indirectly with the human tissues, making it important to evaluate its cytotoxicity. This evaluation can be performed by several methods, but a high discrepancy exists between the approaches used, compromising the reproducibility and the comparison among the obtained results. In this paper, we propose a protocol to evaluate biomaterials cytotoxicity using soluble extracts, which we use for dental biomaterials. The extracts preparation is detailed, from pellets production to its extraction in a culture medium. The biomaterials cytotoxicity evaluation is based on metabolic activity using the MTT assay, cell viability using the Sulphorhodamine B (SBR) assay, cell death profile by flow cytometry, and cell morphology using May-Grünwald Giemsa. Additional to cytotoxicity evaluation, a protocol to evaluate cell function is described based on the expression of specific markers assessed by immunocytochemistry and PCR. This protocol provides a comprehensive guide for biomaterials cytotoxicity and cellular effects evaluation, using the extracts methodology, in a reproducible and robust manner.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Materiais Biocompatíveis
Limite:
Humans
Idioma:
En
Revista:
J Vis Exp
Ano de publicação:
2021
Tipo de documento:
Article