A newly characterized malaria antigen on erythrocyte and merozoite surfaces induces parasite inhibitory antibodies.
J Exp Med
; 218(9)2021 09 06.
Article
em En
| MEDLINE
| ID: mdl-34342640
ABSTRACT
We previously identified a Plasmodium falciparum (Pf) protein of unknown function encoded by a single-copy gene, PF3D7_1134300, as a target of antibodies in plasma of Tanzanian children in a whole-proteome differential screen. Here we characterize this protein as a blood-stage antigen that localizes to the surface membranes of both parasitized erythrocytes and merozoites, hence its designation as Pf erythrocyte membrane and merozoite antigen 1 (PfEMMA1). Mouse anti-PfEMMA1 antisera and affinity-purified human anti-PfEMMA1 antibodies inhibited growth of P. falciparum strains by up to 68% in growth inhibition assays. Following challenge with uniformly fatal Plasmodium berghei (Pb) ANKA, up to 40% of mice immunized with recombinant PbEMMA1 self-cured, and median survival of lethally infected mice was up to 2.6-fold longer than controls (21 vs. 8 d, P = 0.005). Furthermore, high levels of naturally acquired human anti-PfEMMA1 antibodies were associated with a 46% decrease in parasitemia over 2.5 yr of follow-up of Tanzanian children. Together, these findings suggest that antibodies to PfEMMA1 mediate protection against malaria.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Plasmodium falciparum
/
Proteínas de Protozoários
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Malária Falciparum
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Membrana Eritrocítica
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Merozoítos
/
Antígenos de Protozoários
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Child, preschool
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Female
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Humans
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Infant
País/Região como assunto:
Africa
Idioma:
En
Revista:
J Exp Med
Ano de publicação:
2021
Tipo de documento:
Article