Occurrence of Helicobacter spp. and Fecal Bacterial Contamination in High-altitude Aquatic Environments from the Andes.
Bull Environ Contam Toxicol
; 107(3): 433-440, 2021 Sep.
Article
em En
| MEDLINE
| ID: mdl-34347116
ABSTRACT
The occurrence of Helicobacter spp. and fecal bacterial contamination was investigated in high-altitude environments from the Northeastern Andes of Venezuela. Helicobacter DNA was detected by PCR in streams, drinking and irrigation waters, and one culture from drinking water by the HP enrichment medium for selection of Helicobacter pylori, which displayed 98.98% homology to this pathogen based on 16S rRNA gene sequencing. FISH demonstrated predominant coccoid cells of the target bacteria indicative of the viable but nonculturable (VBNC) state in all water samples and HP cultures. Our work reveals for the first time Helicobacter spp. in waters from one of the highest places in the world. These results, together with the presence of fecal coliforms (2-160 MPN/100 mL) from the headwaters of rivers to drinking and irrigation waters, alert fecal contamination and epidemiological implications in this area of ecological and economic importance for the region.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Água Potável
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Helicobacter pylori
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Helicobacter
Idioma:
En
Revista:
Bull Environ Contam Toxicol
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Venezuela