Protocol for isolating young adult parvalbumin interneurons from the mouse brain for extraction of high-quality RNA.

Joseph, Donald J; Von Deimling, Markus; Hasegawa, Yuiko; Cristancho, Ana G; Risbud, Rashmi; McCoy, Almedia J; Marsh, Eric D

Joseph, Donald J; Von Deimling, Markus; Hasegawa, Yuiko; Cristancho, Ana G; Risbud, Rashmi; McCoy, Almedia J; Marsh, Eric D.

Afiliação

Joseph DJ; Division of Child Neurology, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.
Von Deimling M; Division of Child Neurology, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.
Hasegawa Y; Department of Intensive Care Medicine, University Medical Center Hamburg-Eppendorf, Martinistraße 52, 20246 Hamburg, Germany.
Cristancho AG; Division of Child Neurology, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.
Risbud R; Division of Child Neurology, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.
McCoy AJ; Division of Child Neurology, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.
Marsh ED; Division of Child Neurology, Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.

STAR Protoc ; 2(3): 100714, 2021 09 17.

Article em En | MEDLINE | ID: mdl-34401780

Assuntos

Citometria de Fluxo/métodos; Neurônios GABAérgicos/metabolismo; RNA/isolamento & purificação; Animais; Encéfalo/metabolismo; Interneurônios/metabolismo; Espectrometria de Massas/métodos; Camundongos; Parvalbuminas/isolamento & purificação; Parvalbuminas/metabolismo

Palavras-chave

Cell isolation; Flow cytometry/mass cytometry; Molecular biology; Neuroscience

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA / Neurônios GABAérgicos / Citometria de Fluxo Limite: Animals Idioma: En Revista: STAR Protoc Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos

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