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A luminescence-based protocol for assessing fructose metabolism via quantification of ketohexokinase enzymatic activity in mouse or human hepatocytes.
Park, Se-Hyung; Helsley, Robert N; Noetzli, Leila; Tu, Ho-Chou; Wallenius, Kristina; O'Mahony, Gavin; Boucher, Jeremie; Liu, Jianming; Softic, Samir.
Afiliação
  • Park SH; Department of Pediatrics, Division of Pediatric Gastroenterology, University of Kentucky College of Medicine, 900 South Limestone, Wethington Rm 527, Lexington, KY 40536, USA.
  • Helsley RN; Department of Pediatrics, Division of Pediatric Gastroenterology, University of Kentucky College of Medicine, 900 South Limestone, Wethington Rm 527, Lexington, KY 40536, USA.
  • Noetzli L; Alnylam Pharmaceuticals Inc., Cambridge, MA 021428, USA.
  • Tu HC; Alnylam Pharmaceuticals Inc., Cambridge, MA 021428, USA.
  • Wallenius K; Bioscience, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden.
  • O'Mahony G; Medicinal Chemistry, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden.
  • Boucher J; Bioscience, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden.
  • Liu J; The Lundberg Laboratory for Diabetes Research, University of Gothenburg, Gothenburg, Sweden.
  • Softic S; Wallenberg Centre for Molecular and Translational Medicine, University of Gothenburg, Gothenburg, Sweden.
STAR Protoc ; 2(3): 100731, 2021 09 17.
Article em En | MEDLINE | ID: mdl-34409309
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ensaios Enzimáticos / Frutoquinases / Frutose / Medições Luminescentes Tipo de estudo: Guideline Limite: Animals / Humans Idioma: En Revista: STAR Protoc Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ensaios Enzimáticos / Frutoquinases / Frutose / Medições Luminescentes Tipo de estudo: Guideline Limite: Animals / Humans Idioma: En Revista: STAR Protoc Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos