Electrophysiological and behavioral properties of 4-aminopyridine-induced epileptic activity in mice.
Biol Futur
; 71(4): 427-434, 2020 Dec.
Article
em En
| MEDLINE
| ID: mdl-34554464
ABSTRACT
4-aminopyridine (4-AP) is a widely used drug that induces seizure activity in rodents, especially in rats, although there is no consensus in the literature on the dose to be used in mice. The aim of the present study was to investigate the effect of the intraperitoneal administration of 4-AP in two doses (4 and 10 mg/kg) in vivo. EEG, movement, and video recordings were made simultaneously in male B6 mice to specify the details of the seizures and to determine whether there is a suitable non-lethal dose for seizure induction and for further molecular studies. Seizure behavior in mice differs from that seen in rats, with no characteristic stages of epileptic seizures, but with spiking and seizure activity. Seizure activity, although produced at both doses without being lethal, induced different changes of the EEG pattern. Smaller dose induced a lower amplitude seizure activity, decreased spiking activity and later onset of seizures, while higher dose induced a much more intense brain seizure activity and severe trembling. It is concluded that the intraperitoneal administration of 4-AP at a dose of 10 mg/kg induces explicit seizure activity in mice which is repeatable and can be suitable for further molecular research.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
4-Aminopiridina
/
Epilepsia
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Fenômenos Eletrofisiológicos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Biol Futur
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Hungria