Shiga toxin 2eB-transgenic lettuce vaccine: N-glycosylation is important for protecting against porcine edema disease.
J Vet Med Sci
; 83(11): 1708-1714, 2021 Nov 16.
Article
em En
| MEDLINE
| ID: mdl-34556603
ABSTRACT
Porcine edema disease (ED) is a life-threatening toxemia caused by enteric infection with Shiga toxin 2e (Stx2e)-producing Escherichia coli (STEC) in weaned piglets. We previously reported that the stx2eB-transgenic lettuce 2BH strain shows potential for use as an oral vaccine candidate against ED. However, the 2BH strain expressed a hemagglutinin (HA)-tag together with Stx2eB and contained non-canonical N-glycosylation. Therefore, we developed two Stx2eB-lettuce strains, the 3 (G+) strain in which the HA-tag was removed from 2BH, and the 3 (G-) lettuce strain, in which the 73rd Asn was replaced with Ser to prevent non-canonical N-glycosylation of Stx2eB from the 3 (G+) strain. We examined the protective effect of these newly developed two strains compared with the previous 2BH strain against ED using a colostrum-deprived piglet STEC infection model. We found that the N-glycosylated 2BH and 3 (G+) strains relieved the pathogenic symptoms of ED in STEC-challenged piglets, whereas the non-glycosylated 3 (G-) strain did not. N-Glycosylation of the Stx2eB product in lettuce may be involved in the immune response in piglets.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Infecções por Escherichia coli
/
Escherichia coli Shiga Toxigênica
Limite:
Animals
Idioma:
En
Revista:
J Vet Med Sci
Assunto da revista:
MEDICINA VETERINARIA
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Japão