Detection and Imaging of the Plant Pathogen Response by Near-Infrared Fluorescent Polyphenol Sensors.
Angew Chem Int Ed Engl
; 61(2): e202108373, 2022 01 10.
Article
em En
| MEDLINE
| ID: mdl-34608727
ABSTRACT
Plants use secondary metabolites such as polyphenols for chemical defense against pathogens and herbivores. Despite their importance in plant pathogen interactions and tolerance to diseases, it remains challenging to detect polyphenols in complex plant tissues. Here, we create molecular sensors for plant polyphenol imaging that are based on near-infrared (NIR) fluorescent single-wall carbon nanotubes (SWCNTs). We identified polyethylene glycol-phospholipids that render (6,5)-SWCNTs sensitive (Kd =90â
nM) to plant polyphenols (tannins, flavonoids,
), which red-shift (up to 20â
nm) and quench their emission (ca. 1000â
nm). These sensors report changes in total polyphenol level after herbivore or pathogen challenge in crop plant systems (Soybean Glycine max) and leaf tissue extracts (Tococa spp.). We furthermore demonstrate remote chemical imaging of pathogen-induced polyphenol release from roots of soybean seedlings over the time course of 24â
h. This approach allows in situ visualization and understanding of the chemical plant defense in real time and paves the way for plant phenotyping for optimized polyphenol secretion.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polifenóis
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Angew Chem Int Ed Engl
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Alemanha