GC/EI-MS method for the determination of phytosterols in vegetable oils.
Anal Bioanal Chem
; 414(2): 1061-1071, 2022 Jan.
Article
em En
| MEDLINE
| ID: mdl-34716783
ABSTRACT
Sterols are a highly complex group of lipophilic compounds present in the unsaponifiable matter of virtually all living organisms. In this study, we developed a novel gas chromatography with mass spectrometry selected ion monitoring (GC/MS-SIM) method for the comprehensive analysis of sterols after saponification and silylation. A new referencing system was introduced by means of a series of saturated fatty acid pyrrolidides (FAPs) as internal standards. Linked with retention time locking (RTL), the resulting FAP retention indices (RIFAP) of the sterols could be determined with high precision. The GC/MS-SIM method was based on the parallel measurement of 17 SIM ions in four time windows. This set included eight molecular ions and seven diagnostic fragment ions of silylated sterols as well as two abundant ions of FAPs. Altogether, twenty molecular ions of C27- to C31-sterols with 0-3 double bonds were included in the final method. Screening of four common vegetable oils (sunflower oil, hemp oil, rapeseed oil, and corn oil) enabled the detection of 30 different sterols and triterpenes most of which could be identified.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fitosteróis
/
Óleos de Plantas
/
Espectrometria de Massas por Ionização por Electrospray
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Cromatografia Gasosa-Espectrometria de Massas
Tipo de estudo:
Guideline
Idioma:
En
Revista:
Anal Bioanal Chem
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Alemanha