NIR persistent luminescence nanoparticles based turn-on aptasensor for autofluorescence-free determination of 17ß-estradiol in milk.
Food Chem
; 373(Pt A): 131432, 2022 Mar 30.
Article
em En
| MEDLINE
| ID: mdl-34717086
ABSTRACT
Consistent exposure to 17ß-estradiol through drinking water and food can cause health problems. Although many simple and sensitive fluorescence sensors of 17ß-estradiol have been reported, most of them are based on fluorescence quenching test mode working in visible light range, which are inferior in anti-interference ability and quantitative range. Here, we developed a near-infrared (NIR) phosphorescence aptasensor for the detection of 17ß-estradiol that has no background fluorescence. The aptasensor was based on Foster resonance energy transfer (FRET) between aptamer conjugated NIR persistent luminescence nanoparticles (PLNPs-Apt) and MoS2 nanosheets. The 17ß-estradiol was quantified by the recovery of PLNPs' phosphorescence. This assay can detect 17ß-estradiol in 0.5 mL samples with the LOD of 0.29 ng mL-1 and in concentrations of more than three orders of magnitude (from 0.5 ng mL-1 to 1.2 µg mL-1). This aptasensor exhibited selectivity for 17ß-estradiol and was applicable in complex milk samples.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Aptâmeros de Nucleotídeos
/
Nanopartículas
Limite:
Animals
Idioma:
En
Revista:
Food Chem
Ano de publicação:
2022
Tipo de documento:
Article