Semi-Automated High-Throughput Substrate Screening Assay for Nucleoside Kinases.

Hellendahl, Katja F; Fehlau, Maryke; Hans, Sebastian; Neubauer, Peter; Kurreck, Anke

Hellendahl, Katja F; Fehlau, Maryke; Hans, Sebastian; Neubauer, Peter; Kurreck, Anke.

Afiliação

Hellendahl KF; Chair of Bioprocess Engineering, Faculty III Process Sciences, Institute of Biotechnology, Technische Universität Berlin, Ackerstraße 76, 13355 Berlin, Germany.
Fehlau M; Chair of Bioprocess Engineering, Faculty III Process Sciences, Institute of Biotechnology, Technische Universität Berlin, Ackerstraße 76, 13355 Berlin, Germany.
Hans S; BioNukleo GmbH, Ackerstraße 76, 13355 Berlin, Germany.
Neubauer P; Chair of Bioprocess Engineering, Faculty III Process Sciences, Institute of Biotechnology, Technische Universität Berlin, Ackerstraße 76, 13355 Berlin, Germany.
Kurreck A; Chair of Bioprocess Engineering, Faculty III Process Sciences, Institute of Biotechnology, Technische Universität Berlin, Ackerstraße 76, 13355 Berlin, Germany.

Int J Mol Sci ; 22(21)2021 Oct 26.

Article em En | MEDLINE | ID: mdl-34768989

Assuntos

Nucleosídeos/metabolismo; Fosfotransferases/metabolismo; Trifosfato de Adenosina/metabolismo; Animais; Drosophila melanogaster/metabolismo; Avaliação Pré-Clínica de Medicamentos/métodos; Ensaios de Triagem em Larga Escala/métodos; Humanos; Luciferases/metabolismo; Fosforilação/fisiologia; Especificidade por Substrato

Palavras-chave

HPLC; biocatalysis; high throughput; luciferase assay; luminescent assay; nucleoside analogues; nucleoside kinase; nucleotide; robot; screening

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fosfotransferases / Nucleosídeos Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Animals / Humans Idioma: En Revista: Int J Mol Sci Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Alemanha

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