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Exercise Training Protects Against Heart Failure Via Expansion of Myeloid-Derived Suppressor Cells Through Regulating IL-10/STAT3/S100A9 Pathway.
Feng, Lifeng; Li, Guangru; An, Jiale; Liu, Chang; Zhu, Xiaolong; Xu, Yang; Gao, Yang; Li, Jing; Liu, Jie; Yan, Jie; Wang, Yachen; Ren, Jiling; Yang, Liang; Qi, Zhi.
Afiliação
  • Feng L; Department of Molecular Pharmacology, School of Medicine, Nankai University, Tianjin, China (L.F., G.L., J.A., C.L., Y.X., Y.G., J. Li, J. Liu, J.Y., Y.W., L.Y., Z.Q.).
  • Li G; Department of Molecular Pharmacology, School of Medicine, Nankai University, Tianjin, China (L.F., G.L., J.A., C.L., Y.X., Y.G., J. Li, J. Liu, J.Y., Y.W., L.Y., Z.Q.).
  • An J; Department of Molecular Pharmacology, School of Medicine, Nankai University, Tianjin, China (L.F., G.L., J.A., C.L., Y.X., Y.G., J. Li, J. Liu, J.Y., Y.W., L.Y., Z.Q.).
  • Liu C; Department of Molecular Pharmacology, School of Medicine, Nankai University, Tianjin, China (L.F., G.L., J.A., C.L., Y.X., Y.G., J. Li, J. Liu, J.Y., Y.W., L.Y., Z.Q.).
  • Zhu X; Department of Pathogen Biology, Basic Medical College (X.Z., J.R.), Tianjin Medical University, China.
  • Xu Y; Department of Cardiovascular Surgery, Tianjin Chest Hospital, China (X.Z.).
  • Gao Y; Department of Molecular Pharmacology, School of Medicine, Nankai University, Tianjin, China (L.F., G.L., J.A., C.L., Y.X., Y.G., J. Li, J. Liu, J.Y., Y.W., L.Y., Z.Q.).
  • Li J; Department of Molecular Pharmacology, School of Medicine, Nankai University, Tianjin, China (L.F., G.L., J.A., C.L., Y.X., Y.G., J. Li, J. Liu, J.Y., Y.W., L.Y., Z.Q.).
  • Liu J; Tianjin Key Laboratory of General Surgery in Construction, Tianjin Union Medical Center, China (Y.G., L.Y., Z.Q.).
  • Yan J; Department of Molecular Pharmacology, School of Medicine, Nankai University, Tianjin, China (L.F., G.L., J.A., C.L., Y.X., Y.G., J. Li, J. Liu, J.Y., Y.W., L.Y., Z.Q.).
  • Wang Y; Department of Molecular Pharmacology, School of Medicine, Nankai University, Tianjin, China (L.F., G.L., J.A., C.L., Y.X., Y.G., J. Li, J. Liu, J.Y., Y.W., L.Y., Z.Q.).
  • Ren J; Department of Molecular Pharmacology, School of Medicine, Nankai University, Tianjin, China (L.F., G.L., J.A., C.L., Y.X., Y.G., J. Li, J. Liu, J.Y., Y.W., L.Y., Z.Q.).
  • Yang L; Department of Molecular Pharmacology, School of Medicine, Nankai University, Tianjin, China (L.F., G.L., J.A., C.L., Y.X., Y.G., J. Li, J. Liu, J.Y., Y.W., L.Y., Z.Q.).
  • Qi Z; Immunology Department, Key Laboratory of Immune Microenvironment and Disease (Ministry of Education) (J.R.), Tianjin Medical University, China.
Circ Heart Fail ; 15(3): e008550, 2022 03.
Article em En | MEDLINE | ID: mdl-34911348
BACKGROUND: Exercise training (ET) has a protective effect on the progression of heart failure, however, the specific mechanism has not been fully explored. Myeloid-derived suppressor cells (MDSCs) are a group of myeloid-derived immunosuppressive cells, which showed a protective effect in the progression of heart failure. Thus, we hypothesized that the protective effect of ET on heart failure may be related to the infiltration of MDSCs. METHODS: C57BL/6 mice were made to run on a treadmill 6× a week for 4 weeks followed by isoproterenol injection from third week. Heart function was evaluated by echocardiography and the proportion of MDSCs was detected by flow cytometry. Hypertrophic markers, cardiac fibrosis, and inflammatory factors were detected by real-time PCR, ELISA, histological staining, and Western blot. RESULTS: ET treatment in isoproterenol-induced heart failure mice (n=7) enhanced cardiac function (57% increase in FS%, P=0.002) and improved morphological changes compared with isoproterenol mice (n=17). ET further caused 79% increasing in cardiac MDSCs in isoproterenol mice (P<0.001). In addition, depletion of MDSCs by 5-Fluorouracil blunted the cardio-protective effect of ET. T-cell proliferation assay showed that ET did not affect the suppressive activity of MDSCs. Furthermore, we found that ET activated the secretion of IL (interleukin)-10 by macrophages in isoproterenol mice. MDSCs expansion and cardio protection was not present in tamoxifen-inducible macrophage-specific IL-10 knockout mice. Western blot results confirmed that IL-10 regulated the differentiation of MDSCs through the translocation of p-STAT3 (signal transducer and activator of transcription 3)/S100A9 (S100 calcium-binding protein A9) to the nucleus. CONCLUSIONS: ET could increase MDSCs by stimulating the secretion of IL-10 from macrophage, which was through IL-10/STAT3/S100A9 signaling pathway, thereby achieving the role of heart protection.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Condicionamento Físico Animal / Interleucina-10 / Calgranulina B / Fator de Transcrição STAT3 / Células Supressoras Mieloides / Insuficiência Cardíaca Limite: Animals Idioma: En Revista: Circ Heart Fail Assunto da revista: ANGIOLOGIA / CARDIOLOGIA Ano de publicação: 2022 Tipo de documento: Article
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Condicionamento Físico Animal / Interleucina-10 / Calgranulina B / Fator de Transcrição STAT3 / Células Supressoras Mieloides / Insuficiência Cardíaca Limite: Animals Idioma: En Revista: Circ Heart Fail Assunto da revista: ANGIOLOGIA / CARDIOLOGIA Ano de publicação: 2022 Tipo de documento: Article