Characterization of murine A-raf, a new oncogene related to the v-raf oncogene.
Mol Cell Biol
; 6(7): 2655-62, 1986 Jul.
Article
em En
| MEDLINE
| ID: mdl-3491291
ABSTRACT
A 1.6-kilobase cDNA (A-raf) has been isolated from a murine spleen cDNA library which encodes part of a protein related to the raf oncogene. Its amino acid sequence has 85% homology to raf in a central portion of 100 amino acids. In contrast to raf, A-raf shows a highly restricted tissue distribution of expression, with highest levels observed in epididymis, followed by intestine. When incorporated into a retrovirus, the resulting gag-A-raf fusion gene causes transformation in vitro and induces tumors in newborn mice. Thus, A-raf represents a new proto-oncogene. Transformation by A-raf is independent of ras gene function, as is the case for raf and mos but not other oncogenes.
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Base de dados:
MEDLINE
Assunto principal:
Oncogenes
Limite:
Animals
Idioma:
En
Revista:
Mol Cell Biol
Ano de publicação:
1986
Tipo de documento:
Article