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Effects of Different Molecular Weight Polysaccharides From Dendrobium officinale Kimura & Migo on Human Colorectal Cancer and Transcriptome Analysis of Differentially Expressed Genes.
Tao, Shengchang; Ren, Zhiyao; Yang, Zerui; Duan, Shuna; Wan, Zhongxian; Huang, Jiahui; Liu, Chenxing; Wei, Gang.
Afiliação
  • Tao S; School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.
  • Ren Z; Department of Pharmacy, Affiliated Dongguan Hospital, Southern Medical University, Dongguan, China.
  • Yang Z; Department of Systems Biomedical Sciences, School of Medicine, Jinan University, Guangzhou, China.
  • Duan S; NHC Key Laboratory of Male Reproduction and Genetics, Guangzhou, China.
  • Wan Z; Department of Central Laboratory, Family Planning Research Institute of Guangdong Province, Guangzhou, China.
  • Huang J; School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.
  • Liu C; School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.
  • Wei G; Shaoguan Institute of Danxia Dendrobium Officinale, Shaoguan, China.
Front Pharmacol ; 12: 704486, 2021.
Article em En | MEDLINE | ID: mdl-34925000
ABSTRACT
We investigated the antitumor effects of four fractions of Dendrobium officinale Kimura & Migo (D. officinale) polysaccharides with different molecular weights (Mw), Astragalus membranaceus polysaccharides (APS) and Lentinus edodes polysaccharides (LNT) on colorectal cancer (CRC) using a zebrafish xenograft model. Transcriptome sequencing was performed to further explore the possible antitumor mechanisms of D. officinale polysaccharides. Fractions of D. officinale polysaccharides, LNT, and APS could significantly inhibit the growth of HT-29 cells in a zebrafish xenograft model. One fraction of D. officinale polysaccharides called DOPW-1 (Mw of 389.98 kDa) exhibited the strongest tumor inhibition. Compared with the control group, RNA-seq revealed that the DOPW-1-treated experimental group had 119 differentially expressed genes (DEGs), of which 45 had upregulated expression and 74 had downregulated expression. Analyses using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes suggested that the pathway "apoptosis-multiple species" was the most significantly enriched. Our data indicated that 1) fractions of D. officinale polysaccharides of Mw 389.98 kDa were most suitable against CRC; 2) DOPW-1 could be developed into a clinical agent against CRC; and 3) an apoptosis pathway is important for DOPW-1 to inhibit the proliferation of HT-29 cells.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Revista: Front Pharmacol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Idioma: En Revista: Front Pharmacol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China