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Revisiting the role of pulsed electric fields in overcoming the barriers to in vivo gene electrotransfer.
Sachdev, Shaurya; Potocnik, Tjasa; Rems, Lea; Miklavcic, Damijan.
Afiliação
  • Sachdev S; University of Ljubljana, Faculty of Electrical Engineering, Trzaska cesta 25, 1000 Ljubljana, Slovenia.
  • Potocnik T; University of Ljubljana, Faculty of Electrical Engineering, Trzaska cesta 25, 1000 Ljubljana, Slovenia.
  • Rems L; University of Ljubljana, Faculty of Electrical Engineering, Trzaska cesta 25, 1000 Ljubljana, Slovenia.
  • Miklavcic D; University of Ljubljana, Faculty of Electrical Engineering, Trzaska cesta 25, 1000 Ljubljana, Slovenia. Electronic address: damijan.miklavcic@fe.uni-lj.si.
Bioelectrochemistry ; 144: 107994, 2022 Apr.
Article em En | MEDLINE | ID: mdl-34930678
ABSTRACT
Gene therapies are revolutionizing medicine by providing a way to cure hitherto incurable diseases. The scientific and technological advances have enabled the first gene therapies to become clinically approved. In addition, with the ongoing COVID-19 pandemic, we are witnessing record speeds in the development and distribution of gene-based vaccines. For gene therapy to take effect, the therapeutic nucleic acids (RNA or DNA) need to overcome several barriers before they can execute their function of producing a protein or silencing a defective or overexpressing gene. This includes the barriers of the interstitium, the cell membrane, the cytoplasmic barriers and (in case of DNA) the nuclear envelope. Gene electrotransfer (GET), i.e., transfection by means of pulsed electric fields, is a non-viral technique that can overcome these barriers in a safe and effective manner. GET has reached the clinical stage of investigations where it is currently being evaluated for its therapeutic benefits across a wide variety of indications. In this review, we formalize our current understanding of GET from a biophysical perspective and critically discuss the mechanisms by which electric field can aid in overcoming the barriers. We also identify the gaps in knowledge that are hindering optimization of GET in vivo.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Terapia Genética / Técnicas de Transferência de Genes / Eletroporação Limite: Animals / Humans Idioma: En Revista: Bioelectrochemistry Assunto da revista: BIOQUIMICA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Eslovênia

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Terapia Genética / Técnicas de Transferência de Genes / Eletroporação Limite: Animals / Humans Idioma: En Revista: Bioelectrochemistry Assunto da revista: BIOQUIMICA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Eslovênia