Association of RanGAP to nuclear pore complex component, RanBP2/Nup358, is required for pupal development in Drosophila.
Cell Rep
; 37(13): 110151, 2021 12 28.
Article
em En
| MEDLINE
| ID: mdl-34965423
Ran's GTPase-activating protein (RanGAP) is tethered to the nuclear envelope (NE) in multicellular organisms. We investigated the consequences of RanGAP localization in human tissue culture cells and Drosophila. In tissue culture cells, disruption of RanGAP1 NE localization surprisingly has neither obvious impacts on viability nor nucleocytoplasmic transport of a model substrate. In Drosophila, we identified a region within nucleoporin dmRanBP2 required for direct tethering of dmRanGAP to the NE. A dmRanBP2 mutant lacking this region shows no apparent growth defects during larval stages but arrests at the early pupal stage. A direct fusion of dmRanGAP to the dmRanBP2 mutant rescues this arrest, indicating that dmRanGAP recruitment to dmRanBP2 per se is necessary for the pupal ecdysis sequence. Our results indicate that while the NE localization of RanGAP is widely conserved in multicellular organisms, the targeting mechanisms are not. Further, we find a requirement for this localization during pupal development.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Pupa
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Chaperonas Moleculares
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Regulação da Expressão Gênica no Desenvolvimento
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Proteínas Ativadoras de GTPase
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Poro Nuclear
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Complexo de Proteínas Formadoras de Poros Nucleares
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Proteínas de Drosophila
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Drosophila melanogaster
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Membrana Nuclear
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Cell Rep
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Estados Unidos