Media optimization for SHuffle T7 Escherichia coli expressing SUMO-Lispro proinsulin by response surface methodology.

Khalilvand, Aida Bakhshi; Aminzadeh, Saeed; Sanati, Mohammad Hossein; Mahboudi, Fereidoun

Khalilvand, Aida Bakhshi; Aminzadeh, Saeed; Sanati, Mohammad Hossein; Mahboudi, Fereidoun.

Afiliação

Khalilvand AB; Bioprocess Engineering Group, Institute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Aminzadeh S; Bioprocess Engineering Group, Institute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran. aminzade@nigeb.ac.ir.
Sanati MH; Medical Genetics Group, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Mahboudi F; Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.

BMC Biotechnol ; 22(1): 1, 2022 01 03.

Article em En | MEDLINE | ID: mdl-34980082

Assuntos

Escherichia coli; Proinsulina; Meios de Cultura/química; Dissulfetos; Escherichia coli/genética; Escherichia coli/metabolismo; Fermentação; Insulina Lispro/metabolismo; Proinsulina/genética

Palavras-chave

CCD; E. coli; Medium composition; PBD; RSM; SHuffle

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proinsulina / Escherichia coli Idioma: En Revista: BMC Biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Irã

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