Immunofluorescence-Based Method to Assess Cancer Biomarker in the Hypoxic Region of the Tumor.
Methods Mol Biol
; 2413: 37-43, 2022.
Article
em En
| MEDLINE
| ID: mdl-35044652
ABSTRACT
The development of novel imaging technologies allows the analysis of the expression and spatial distribution of multiple markers simultaneously, providing necessary information about a cellular identity and the surrounding microenvironment. This chapter describes the utilization of immunofluorescence to identify such biomarkers in fixed tissue from prostate cancer (PCa) xenografts. One such marker detectable by immunofluorescence is pimonidazole, which has been utilized to locate areas of low oxygen (hypoxia). Pimonidazole, in combination with other biomarkers, could be utilized to identify "niches" in the microenvironment harboring more aggressive cells both within and outside hypoxic areas. Specifically, we describe the method to use pimonidazole for the identification of hypoxic regions in PCa xenograft tumors along with CPT1A (carnitine palmitoyltransferase 1A) expression, an indicator of ß-oxidation. This approach could be useful to characterize various biomarkers in the complex hypoxic tumor microenvironment.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Neoplasias
/
Nitroimidazóis
Limite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Estados Unidos