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Effect of heterogeneous substrate adhesivity of follower cells on speed and tension profile of leader cells in primary keratocyte collective cell migration.
Chakraborty, Madhura; Mukherjee, Baishali; Nalinakshan, Nanditha; Biswas, Arikta; Nayak, Rajesh Kumble; Sinha, Bidisha.
Afiliação
  • Chakraborty M; Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Mohanpur, Nadia 741246, India.
  • Mukherjee B; Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Mohanpur, Nadia 741246, India.
  • Nalinakshan N; Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Mohanpur, Nadia 741246, India.
  • Biswas A; Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Mohanpur, Nadia 741246, India.
  • Nayak RK; Department of Physical Sciences, Indian Institute of Science Education and Research Kolkata, Mohanpur, Nadia 741246, India.
  • Sinha B; Center of Excellence in Space Sciences, India, Indian Institute of Science Education and Research Kolkata, Mohanpur, Nadia 741246, India.
Biol Open ; 11(3)2022 03 15.
Article em En | MEDLINE | ID: mdl-35146504
ABSTRACT
In single keratocyte motility, membrane tension is reported to be high at cell-fronts and believed to establish front coherence. To understand role of membrane mechanics in collective cell migration, we study membrane height fluctuations in cell sheets from fish scales using interference reflection microscopy (IRM). We report the monolayer to have cells lacking substrate adhesion and show that such 'non-sticky' cells can form bridges between leader cells and far-away follower cells. Do such interactions alter motility and membrane mechanics in such leaders? We find non-significant, but reduced speed for leaders with 'non-sticky' followers in comparison to other leaders. Cells show high phenotypic variability in their membrane fluctuation tension profiles. On average, this tension is found to be lower at cell fronts than the mid-section. However, leaders with non-sticky followers are more prone to display higher tension at their front and have a negative correlation between cell speed and front-mid tension difference. Thus, we conclude that intracellular tension gradients are heterogeneous in cell sheets and substrate adhesivity of followers can control the coupling of the gradient to cell speed.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Adesivos / Peixes Limite: Animals Idioma: En Revista: Biol Open Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Índia

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Adesivos / Peixes Limite: Animals Idioma: En Revista: Biol Open Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Índia