Efficient Bioproduction of Human Milk Alpha-Lactalbumin in Komagataella phaffii.
J Agric Food Chem
; 70(8): 2664-2672, 2022 Mar 02.
Article
em En
| MEDLINE
| ID: mdl-35148078
ABSTRACT
Alpha-lactalbumin (α-LA; the most abundant whey protein in human milk) contributes to infant development, providing bioactive peptides and essential amino acids. Here, Komagataella phaffii (K. phaffii) was selected as the production host. We found that the K. phaffii host X33 was suitable for expressing the target protein, yielding 5.2 mg·L-1 α-LA. Thereafter, several secretory signal peptides were applied to obtain a higher titer of α-LA. The strain with α-factor secretory signal peptide secreted the highest extracellular titer. Additionally, promoters AOX1, GAP, and GAP(m) were compared and applied. The strain with the promoter AOX1 produced the highest extracellular titer. In addition, coexpressing human protein disulfide isomerase A3 (hPDIA3) increased the titer by 27%. Human α-LA production by the strain X33-pPICZαA-hLALBA-hPDIA3 reached 56.3 mg·L-1 in a 3 L bioreactor. This is the first report of successful secretory human α-LA expression in K. phaffii and lays foundations for the simulation of human milk for infant formulas and further development of bioengineered milk.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Saccharomycetales
/
Lactalbumina
Limite:
Child
/
Humans
Idioma:
En
Revista:
J Agric Food Chem
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
China