Your browser doesn't support javascript.
loading
E-Protein Inhibition in ILC2 Development Shapes the Function of Mature ILC2s during Allergic Airway Inflammation.
Barshad, Gilad; Webb, Lauren M; Ting, Hung-An; Oyesola, Oyebola O; Onyekwere, Oluomachi G; Lewis, James J; Rice, Edward J; Matheson, Macy K; Sun, Xiao-Hong; von Moltke, Jakob; Danko, Charles G; Tait Wojno, Elia D.
Afiliação
  • Barshad G; Baker Institute for Animal Health, Cornell University College of Veterinary Medicine, Ithaca, NY.
  • Webb LM; Department of Biomedical Sciences, Cornell University College of Veterinary Medicine, Ithaca, NY.
  • Ting HA; Department of Immunology, University of Washington, Seattle, WA; lwebb2@uw.edu etwojno@uw.edu.
  • Oyesola OO; Department of Immunology, University of Washington, Seattle, WA.
  • Onyekwere OG; Department of Immunology, University of Washington, Seattle, WA.
  • Lewis JJ; Baker Institute for Animal Health, Cornell University College of Veterinary Medicine, Ithaca, NY.
  • Rice EJ; Department of Microbiology and Immunology, Cornell University College of Veterinary Medicine, Ithaca, NY; and.
  • Matheson MK; Baker Institute for Animal Health, Cornell University College of Veterinary Medicine, Ithaca, NY.
  • Sun XH; Department of Biomedical Sciences, Cornell University College of Veterinary Medicine, Ithaca, NY.
  • von Moltke J; Baker Institute for Animal Health, Cornell University College of Veterinary Medicine, Ithaca, NY.
  • Danko CG; Department of Biomedical Sciences, Cornell University College of Veterinary Medicine, Ithaca, NY.
  • Tait Wojno ED; Department of Immunology, University of Washington, Seattle, WA.
J Immunol ; 208(5): 1007-1020, 2022 03 01.
Article em En | MEDLINE | ID: mdl-35181641
E-protein transcription factors limit group 2 innate lymphoid cell (ILC2) development while promoting T cell differentiation from common lymphoid progenitors. Inhibitors of DNA binding (ID) proteins block E-protein DNA binding in common lymphoid progenitors to allow ILC2 development. However, whether E-proteins influence ILC2 function upon maturity and activation remains unclear. Mice that overexpress ID1 under control of the thymus-restricted proximal Lck promoter (ID1tg/WT) have a large pool of primarily thymus-derived ILC2s in the periphery that develop in the absence of E-protein activity. We used these mice to investigate how the absence of E-protein activity affects ILC2 function and the genomic landscape in response to house dust mite (HDM) allergens. ID1tg/WT mice had increased KLRG1- ILC2s in the lung compared with wild-type (WT; ID1WT/WT) mice in response to HDM, but ID1tg/WT ILC2s had an impaired capacity to produce type 2 cytokines. Analysis of WT ILC2 accessible chromatin suggested that AP-1 and C/EBP transcription factors but not E-proteins were associated with ILC2 inflammatory gene programs. Instead, E-protein binding sites were enriched at functional genes in ILC2s during development that were later dynamically regulated in allergic lung inflammation, including genes that control ILC2 response to cytokines and interactions with T cells. Finally, ILC2s from ID1tg/WT compared with WT mice had fewer regions of open chromatin near functional genes that were enriched for AP-1 factor binding sites following HDM treatment. These data show that E-proteins shape the chromatin landscape during ILC2 development to dictate the functional capacity of mature ILC2s during allergic inflammation in the lung.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Asma / Fatores de Transcrição / Linfócitos T / Antígenos de Dermatophagoides / Proteína 1 Inibidora de Diferenciação Limite: Animals Idioma: En Revista: J Immunol Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Asma / Fatores de Transcrição / Linfócitos T / Antígenos de Dermatophagoides / Proteína 1 Inibidora de Diferenciação Limite: Animals Idioma: En Revista: J Immunol Ano de publicação: 2022 Tipo de documento: Article